抄録
The initial stage of virus-cell interaction was studied in a system of PR8 virus and Earle's L cell. Used virus was highly labelled with P32 and not contaminated more than 5% of P32 other than virus.
In conducting the experiment, these 2 particles, i.e. virus and host cell were combined and agitated for 2 hrs. at 37°C. Then the cells were removed from the mixture by centrifugation and the nature of hemagglutinins and distribution of radioactivity in this supernatant were compared to those in the inoculum.
Hemagglutinin titer left in the supernatant has been proved to increase after the incubation, particularly when tested with guinea pig red cells. Furthermore, the P32 count detectable after red cell adsorption or after ultracentrifugation which will be enough to sediment elementary bodies, has been increased. Part of them was sedimented with small hemagglutinins and easily extracted with ether, but at 37°C. Moreover, some radioactivity disappeared from the supernate to the L cell debris. Thus the disintegration of virus particles after contact with L cell was suggested.
