Abstract
From in vivo induced tumors by adenovirus type 12, four cell lines were established in vitro (H-1, H2-1, H2-2 and H-4) and from the in vitro transformed foci in embryonal hamster brain tissue culture by the same virus, 7 lines were successfully subcultivated (HT-1, HT-2 so on). There was no gross differences in their characteristics. Morphologically they were tenaciously adherent small epithelioid cells. They needed large inoculum to subculture in rubber stoppwred culture system. Cellular aggregations were frequently observed in the static culture conditions and calcium concentration suggested to bear it. The attempt to isolate the infectious virus from tumor cells and transformed cells at various passage levels has failed so far.
