Method of Separation and Identification of Proteases by Electrophoresis on Cellulose Acetate Membrane

Abstract

A method of separation and identification of proteases by electrophoresis on cellulose acetate membrane is presented.
Proteases were separated by electrophoresis on cellulose acetate membrane. The membrane was layered onto a substrate-agar plate and incubated. The proteolysis areas were distinguished from the non-digested back-ground by direct observation or by protein staining.
The electrophoretic localization of proteases tested were as follows:
Trypsin (×3 crystallized): post-γ globulin fraction (1)
Trypsin (commercial): α-2, β and post-γ globulin fractions (3)
Chymotrypsin: post-γ globulin fraction (1)
Bromelain: post-γ globulin fraction (1)
Pronase: post-γ globulin fractions (3)
Urokinase: diffuse digestion between α-2 and post-β globulin fractions (3)
Plasminogen in serum: α-2 and β globulin fractions (2)
Figures in parentheses indicate the number of lysis area.