Histochemical Demonstration of Glycogen Synthetase in the Normal Human Epidermis

Abstract

A method for histochemieal demonstration of glycogen synthetase activity in the human epidermis was examined. For microscopically discerning glycogen synthetase reaction at the tissue site, a concentration of uridine diphosphate glucose about 4 times that in the Takeuchi and. Glenner's substrate medium was required. With the increase of glucose-6-phosphate concentration, the incubation time necessary for the appearance of reaction was shortened. This effect was most prominent at pH 9.0. Based on these results, the optimum composition of the substrate medium was described.