日本冷凍空調学会論文集
Online ISSN : 2185-789X
Print ISSN : 1344-4905
ISSN-L : 1344-4905
研究レビュー
ビトリフィケーション
氷晶形成なしの-196°C低温保存
高橋 恒夫
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ジャーナル フリー

1993 年 10 巻 2 号 p. 165-173

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Vitrification is an alternative to customary approaches to cryopreserve cell, tissue and organ. In this method, ice formation can be prevented by a combination of high solute concentration and rapid cooling, a solution become glassy without ice crystalline formation at temperatures below-115°C. The cell and tissue damage associated with ice formation is avoided, but thawing should be rapid enough to prevent ice growth during warming and they should be equilibrated with the vitrification medium without injury.
This approach has been extensively studied in the past few years, and has the potential to be an alternative approach to the cryopreservation of a wide range of biological systems.
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© 1993 公益社団法人 日本冷凍空調学会
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