In this paper the effects of flow separation both on the freezing heat transfer characteristics and on the pressure drop of a duct flow have been investigated. Under some condition of temperature and flow separation an ice-water interface in the presence of a turbulence has been observed to be complexed. The degree of flow separation was varied by employing the orifices of various heights at the inlet of the duct. In this study, the range of Reynolds number ReH coveredfrom 2.61×103 to 1.36×l04 and cooling temperature ratios to the water temperature from 2.0 to 30.0. Velocity profile and turbulence intensity distribution in the flow passage were measured using a Laser Doppler Velocimeter. The measurement results showed that the first and the second space of ice layer were strongly related to the flow separation by an orifice and that the stepped ice pattern after 2nd spacing ice-bands was very similar to the patterns which was observed in the case of pie flow.