低温粘着フィルムを利用した凍結魚肉内氷結晶観察法

抄録

In this study, an adhesive film method was newly applied to observe ice crystal morphology of frozen fish meat. A frozen fish meat was embedded with OCT compound and sectioned by use of a cryomicrotome. During the sectioning process, a cryo-adhesive film was used to collect the sections. Then, the section was fixed and stained on the cryo-adhesive film. These processes enabled facile and rapid observation of ice crystals by microscope. With measurement of ice crystal morphology, it was found that ice crystals observed with this applied method were about the same size and shape as those of the freeze-substitution method. Those results demonstrate that this applied method is useful to the observation of ice crystals. Moreover, by use of sequential sections obtained by this method, three-dimensional image of ice crystals in frozen fish meat was easily reconstructed.