抄録
Changes in the membrane ultrastructure after freezing in protoplasts isolated from non-acclimated or cold-acclimated rye (Secale cereale L. cv. Puma) leaves or in protoplasts fused with liposomes were examined with freeze-fracture electron microscopy. In non-acclimated protoplasts, freezing to -10℃ or lower resulted in survival decrease below 10% and induced several changes in the ultrastructure of the plasma membrane and endomembranes of protoplasts. In acclimated protoplasts, these changes were not observed even at -25℃, though protoplasts were injured at this temperature. Fusion of non-acclimated protoplasts with liposomes composed of dilinoleoylphosphatidylcholine mitigated or eliminated the changes in the plasma membrane. Possible roles of membrane lipids on the changes in the membrane ultrastructure induced by freezing were discussed.
