患者由来腸管オルガノイドを用いた安全性評価への応用

抄録

One of the most exciting advancements in stem cell research of the past decade has been the development of organoid systems. The novel method for the long-term culture of isolated intestinal crypts in a three-dimensional (3D) extracellular matrix.

This 3D model system of intestinal-derived organoid culture exhibits the key features of adult intestinal epithelium, including a crypt-villus morphology, a functional central lumen and all of the major cell types. As these cultures better represent the physiology of the intestinal epithelium than immortalized or transformed cell lines (e.g. Caco-2, MC38) and are not associated with the same costs and ethical considerations as in vivo experiments, organoid cultures may serve as useful screening tools during drug development.

Intestinal organoids derived from patient samples have been used to screen patient drug responses in vitro before administering treatment to direct the care and predict therapeutic outcomes of cancer and cystic fibrosis patients.

Here, we introduce the advanced intestinal organoids long-term culture system and the promising aspect of intestinal organoids as a model for the application of drug safety research from patient-derived tissue samples.