Allergic contact dermatitis is caused by substances such as plants, animals, medicines, metals and foods, and the skin barrier dysfunction causes the skin symptoms such as drying, itching and red flare. It was generally difficult to monitor the status of drying and itching. In order to quantify the skin function and scratching behavior and to determine the status of symptoms, we tried to develop the evaluation method for the drying and itching by atopic dermatitis model in hairless mice.
To produce symptoms similar to atopic dermatitis, female Hos: HR-1 hairless mice (Japan SLC Inc.) were fed a special diet (HR-AD) and applied the mite antigen ointment (Biostir AD®) to induce itching and drying. Before and after induction, skin moisture content, TEWL (transepidermal water loss) and viscoelasticity were measured by a Cutometor MPA580 (skin function measurement device), and scratching behavior was analyzed using the animal motion analysis system (ANIMA). Skin inflammation scoring, IgE concentration in the blood, cytokine (multiplex method) in the auricle related to inflammatory response, and histopathological findings were quantitatively evaluated the correlation with skin function and scratching. In addition, the improvement effect of tacrolimus on the skin symptoms was investigated by this quatification method.
In this conference, we will report not only this study result also the result comparing with “the skin toxicity evaluation of atopic dermatitis in NC / Nga mice” published last year.
