Abstract
For the determination of pteroylglutamic acid excreted in the 24 hour urine after an oral dose of 10 mg of pteroylglutamic acid, a simple and rapid method was reported, which was a modification of Allfrey et al.'s. In this mothod, pteroylglutamic acid in the urine was precipitated with lead acetate and eluated in ammonium sulfate solution, then oxidized by potassium permanganate, and finally meassured fluorometrically. The pteroylglutamic acid added to urine was recovered satisfactorily by this method.
