人赤血球, 人赤血球礎質および酵母細胞によるビタミンB_1ならびに諸種ビタミンB_1誘導体の摂取について

抄録

Comparative studies were made on the ingestion of thiamine and its derivatives by human erythrocytes, erythrocyte stroma, and yeast cells. The experiments were carried out in vitro, and S, S-alkyl, O, S-diacyl, O-acyl, S-alkyl, and various other derivatives were tested. 1) First, the intensity of the thiochrome reaction of thiamine analogues was compared with that of thiamine using the cyanogen bromide method. 2) Next, using thiamine propyldisulfide the conditions suitable for comparing the ingestion of various derivatives by erythrocyte were determind. 3) Then the ingestion of various derivatives by erythrocyte was compared. The order of the incorporation degree was as follows : S, S-alkyl derivatives>S, S-alkyl-O-acyl>O, S-diacyl>thiamine>O-acyl. 4) The incorporation of the derivatives into erythrocyte stroma was also examined. In this case, remarkable differences such as in the case of erythrocyte were not observed. 5) The ingestion of the derivatives by yeast cells was examined. The appearence of the incorporation and the order of the incorporation degree of the compounds were considerably different from those in the case of erythrocyte. 6) The mechanism of the ingestion of thiamine derivatives through cell membrane was discussed.