抄録
The amount of thiamine degraded was determined in a system, in which both thiaminase I and II simultaneously existed. It was found that about the same amount of thiamine was degraded, in the presence of the both culture supernatant, aniline or pyridine, as that by thiaminase I alone. Accumulative action of both thiaminases was never observed. On the other hand, when thiamine was degraded by the combined suspension of washed cells of both types producing thiaminase I and II with about equal activity, the amount of thiamine degraded was largely dependent of thiaminase II. A definite accumulative action was scarcely observed. The centrifuged supernatant of Bacillus thiaminolyticus Matsukawa et Misawa was found to have antibacterial activity against Escherichia coli whereas that of Bacillus aneurinolyticus Kimura et Aoyama failed to show the activity. The possible infection mechanism of Bacilluus thiaminolyticus in human intestine was discussed.
