抄録
The optimum pH of thiaminase obtained from the culture broth or the bacterial cell of Bacillus thiaminolyticus was 6.0 and that of Clostridium thiaminolyticus was 7.5. The decomposition products of thiamine by these two intracellular enzymes were shown to be identical. When the culture of B.thiaminolyticus grew older on the agar plate, the enzymatic activity decreased in proportion to the decrease of vegetative cells of the bacteria.
