抄録
Thiamine synthesis from pyrimidine- and thiazole moieties by washed cells of several strains of Enterobacteriaceae was studied. In the case of Escherichia coli ATCC 9637,a good yield of thiamine was obtained when the reaction was carried out under an aerated condition in the presence of glucose. The synthesized vitamin was almost exclusively in esterified form and was practically restricted intracellularly. Among 3 strains of thiamine requireing mutants derived from E.coli ATCC 9637,only 70-23,which required intact thiamine, failed to synthesize an appreciable amounts. Strains 70-17 and 26-43,each of which was capable of growing in the presence of pyrimidine- or thiazole moiety respectively, synthesized much more amount of thiamine than their parent strain. All but one (a strain of Shigella dysenteriae 1) of strains examined which belong to Shigella or Alkalescens・Dispar groups were also found to synthesize thiamine from pyrimidine- and thiazole moieties. The yield of the vitamin was greatly improved by the aeration of the reaction mixture.
