The effects of vitamins B_6,thiamine and riboflavin on the active absorption of amino acid from the intestin were examined. Absorption of alanine was parallel to the vitamin B_6 content of the liver and was reduced in vitamin B_6 deficiency and was restored in the recovering stage. Inhibition of absorption was found in administration of a vitamin B_6 antagonist and it was prevented by administration of pyridoxine. In thiamine and riboflavin deficiency, active absorption of alanine from the intestine increased irrespective of the vitamin B_6 content of the organs. Administration of thiamine antagonist resulted in a decrease in oxygen consumption of the intestine as well as a decrease in absorption and this reduction was prevented only by administration of thiamine.