抄録
It was reconfirmed that the amounts of thiamine in leaves and stems of rice plant were increased in proportion to growth of the plant in water culture. Thiamine content of soybean seedlings was greatly increased when seeds were soaked for short time in the buffer solution containing 2-methyl-4-amino-5-methylhydroxypyrimidine and thiazole moiety and then were allowed to germinate in the light. And also, if the pyrimidine and thiazole moieties were adhered on the leaves of rice plant, barley and beans, the vitamin contents in these plants raised gradually and were reverted again to each original content. These findings suggest that the both moieties were utilized for the biogenesis of the vitamin in plant in vivo. The existence of biosynthetic system of the vitamin in which the both moieties condensed enzymatically in the presence of ATP and Mg ion, however, could not be demonstrated in vitro using the homogenates of various sources of plant.