抄録
On incubation of TATD with heparinized blood of a rabbit, thiamine liberation was observed by paper partition chromatography. Liberation of another counterpart, the lipoate moiety, was investigated bioautographically after the paper electrophoresis. Mobility of an active band which was detected after the incubation was identical with that of lipoic acid. These results show reductive splitting of disulfide linkage in TATD and hydrolysis of ester bond of lipoate moiety by the blood preparation. TATD reducing activity of rabbit blood was present predominantly in blood cell and the apparent ratio of the activity of blood cell to plasma was calculated to be about 12. The hydrolyzing activity of rabbit blood to methyl dihydrolipoate was found in both of blood cell and plasma in about same degree. Presence of deacetylation activity for S-acetyl derivative of dihydrolipoic acid in rabbit blood preparation was ascertained by means of the method of Lipmann and Tuttle. These results indicate that TATD is restored to its original components, thiamine and dihydrolipoic acid (or lipoic acid), in rabbit blood.
