Abstract
The influences of massive dose of thiamine propyldisulfide (TPD) on the growth of embryonic nerve of chick, in vitro, the functional recovery related to adult neural regeneration of rabbit, in vivo, and on the prevention of progressive neuropathy owing to acrylamide poisoning were investigated. The growth of axon from spinal ganglion in 11th day's chick embryo was promoted 1.5 times of the control by 6.25 μg per ml of TPD at 72 hours incubation. The effects were not recognized by the application of thiamine or cocarboxylase. Accompanying a process of the regeneration of amputated sciatic nerve in adult rabbits, electromyogram pattern become to indicate the recovered nerve function. With daily injection of TPD 10 mg per head, the occurances of these patterns were all accelerated and further the amplitude of each pattern was obviously higher than that of control. Ataxia resulted from acrylamide polyneuritis in rabbits was delayed remarkably by daily administration of TPD 20 mg per head. Utilizing contact autoradiography, ^<35>S- and ^<14>C-TPD (inner) were taken closely in motor neuron of spinal cord. In above results, it could be assumed that the application of massive dose of TPD in vitro or in vivo promotes the biosynthesis of nucleoprotein in neuron correlated with axonal protein.
