抄録
The present paper deals with the experiments on the isolation and identification of riboflavin compounds of glucosyl oligosaccharide produced by the action of extracellular enzyme prepared from Leuconostoc mesenteroides NRRL B-1299. By the multiple paper chromatography in a macroscale, seven kinds of riboflavin compounds of glucosyl oligosaccharide were separated, and they were revealed chemically and enzymatically to be 5'-D-riboflavin-α-D-glucopyrano-side, two kinds of riboflavindisaccharides (kojibioside-like substance and isomaltoside), two kinds of riboflavintrisaccharides (glucosyltrisaccharide and dextrantrioside), riboflavin tetrasaccharide and riboflavinpentasaccharide, respectively. By the action of extracellular enzyme, moreover, riboflavin compounds of higher members of glucosyl oligosaccharide were obtained through successive transfer of glucosyl group between sucrose and riboflavin compounds of glucosyl oligosaccharide.
