Effects of pyridoxine deficiency on carbohydrate metabolism were investigated in albino rats and the following results were obtained. 1) aldose-^<14>C and ketose-^<14>C were oxidized more readily in pyridoxine deficient rats than in control rats at 30 to 60 minutes after injection. 2) Pyridoxine deficient rats contained more glycogen in liver and muscles after being fed and less when fasting, as compared to the control. 3) Incorporation of glucose into fat was normal but that of acetate was impaired. 4) Disappearance of injected glucose from serum was delayed in pyridoxine deficient rats. Gluconeogensis, however, was not accelerated. 5) Increased oxidation of aldose and ketose was not influenced by insulin, glucagon, hydrocortisone and epinephrine. Significance of these findings in turns of energy metabolism in pyridoxine deficiency was discussed. Some of the vitamin B_6 analogues also elicited the increased oxidation of glucose.
