STUDIES ON THE EFFECT OF VITAMIN E UPON THE PRESERVATION OF BLOOD : (IV) THE EFFECT OF α-TOCOPHERYLGLYCOLATE ON THE OSMOTIC FRAGILITY OF ERYTHROCYTES AND LEUCOCYTES

Abstract

The influence of vitamin E on the osmotic fragility of erythrocyte and leucocyte counts was investigated. The blood samples were obtained from normal persons in citrate・phosphate・dextrose solution or in EDTA-K_3. α-Tocopherylglycolate was added with the concentration of 20 or 50 mg%. The osmotic fragility was measured by Dacie's method and leucocyte count by micro cell counter, once or twice a week for 3 to 4 weeks. The osmotic fragility was evidently increased and leucocyted and leucocytes showed decrease in control and tocopherol groups, but it was less marked in the latter. The protective potency of the increase of osmotic fragility was more marked in 50 mg % than 20 mg % of α-tocopherylglycolate, and its potency was almost equal comparing with that of α-tocopherylacetate.