抄録
In the previous report we have presented evidence which indicates that in E. coli the free form of thiamine is not involved in de novo synthesis of TPP, but TMP is directly phosphorylated to form TPP and that exogenous thiamine is converted to TPP via the intermediate formation of TMP. Further experiments including the growth responses and TDP-forming abilities of both TMP- and TDP-requiring mutants have been carried out. These results have reaffirmed the previous conclusions.
