ビタミンD受容体リガンドの生体内可視化を目指した分割型ルシフェラーゼバイオセンサーの開発（特集『ビタミン・バイオファクター研究の新潮流』 （第72回大会若手シンポジウム））

抄録

In vivo imaging is analyzing methods to visualize protein-protein interaction and ligand-protein interaction in living cells or animals using fluorescence or luminescence. The split-luciferase technique which is one of the in vivo imaging tools has been used to perform the real-time imaging of many molecules. Vitamin D receptor (VDR) ligands controls the expression of numerous genes via binding to VDR. However, spatiotemporal action of VDR ligands is unclear in living individuals. In the past, we developed the detection tool of VDR ligands using the split-luciferase technique called vitamin D biosensor. In future, vitamin D biosensor is expected as an analyzing tool to elucidate the entirety of vitamin D in vivo.