高速液体クロマトグラフ法による医薬品の分析(第10報)製剤中のアクリノールおよび塩化ベルベリンの同時定量
1980 年 100 巻 7 号 p. 766-770
詳細
1980 年 100 巻 7 号 p. 766-770
The simultaneous determination of acrinol (6, 9-diamino-2-ethoxyacridine monolactate) and berberine chloride by ion-paired high-speed liguid chromatography is described. Separation can be achieved within 15 min, employing a 25 cm column packed with Zorbax ODS, using 5 mM sodium lauryl sulfate in 95% acetonitrile as an eluent with a Shimadzu Du pont LC-3A liquid chromatograph. The accuracy of this method was found to ±1%. Acrinol and berberine chloride in pharmaceutical preparations were examined by this analytical method. These components was extracted with 20% (v/v) acetic acid and the extract was injected directly into the column. The contents was calculated from the calibration curve of previously prepared standards. This method is considered to be useful for the evaluation of these drugs.
