ポリカーボネート膜により順次濾過したリポソームの粒子径減少と準弾性光散乱法

抄録

Sizes of sequentially extruded multilamellar liposomes were measured by a method of quasi elastic laser light scattering (QELS method). Sequential extrusion was started with 1.0 μm filter, then 0.8, 0.6, 0.4, 0.2, and finally with 0.08 μm filters. Polydispersity and skewness of size distribution were analyzed by a cumulative expansion method. Mean diameter, polydispersity, and skewness decreased due to the sequential extrusion through polycarbonate membrane filters. Effects of the addition of cholesterol into lecithin on these physico-chemical parameters were also studied. Liposome size was decreased by the extrusion until with 0.4 μm filter. But further sequential extrusion did not induce a large decrease in the size, suggesting that the most liposomes pass through the pore by changing their shape.