1983 年 103 巻 2 号 p. 245-249
To characterize and identify hetero-aromatic compounds systematically, the liquid chromatographic retention behavior of 19 pyridine and pyrazine derivatives was investigated using various packing materials. A linear correlation between the logarithms of capacity ratio and the logarithms of binary solvent composition was obtained for weaker polar solutes and solvents by silica gel liquid-solid chromatography. Reversed-phase (RP) systems were required for the separation of higher polar solutes such as pyrazine N, N-dioxides. A non linear correlation between retention indices and the methanol-water composition was observed for octadecylsilyl-silica packing. This suggests a complex retention mechanism including the participation of a silanol group on the silica gel surface. On the other hand, an ideal linear correlation between the logarithm of the capacity ratio and solvent composition was obtained in the RP system using polystyrene (PS) gel. The PS gel reversed-phase system seems to be involved in a simpler retention mechanism than the chemically bonded-silica gel phase system and consequently more suited for the determination of basic nitrogen compounds.