酵素阻害活性による漢方処方の検討(第7報)Adenosine 3', 5'-Cyclic Monophosphate Phosphodiesteraseによる菌〓蒿湯の研究

抄録

A Chinese herbal medicinal prescription, Inchinko-to, was studied for the inhibitory activity on adenosine 3', 5'-cyclic monophosphate (cAMP) phosphodiesterase (PDE). The inhibitory activity on cAMP PDE of the extract of each constituent from the crude drug and of several compounds isolated from the drug was studied. By changing the combination ratio of two kinds of the crude drugs from 1 : 1 to 1 : 5, the inhibitory activity on cAMP PDE was estimated and it was found to be highest at the ratio of 1 : 3 in Rhubarb and Gardenia fruit, and at 1 : 4 in Rhubarb and Capillaris buds. These were the same ratio which is now commonly used for Chinese herbal medicinal prescription in Japan. Similar results were obtained in the combination test of the components of the crude drug such as emodin, geniposide, capillarisin and dimethylesculetin. The effects of Inchinko-to and its prescription which lacks one of the constituents of the crude drugs on carbon tetrachloride inducing liver injury in mice were also studied. Among the four prescriptions the antihepatotoxic activities were observed in the prescriptions containing Capillaris buds. Correlation between the administrated content of dimethylesculetin and antihepatotoxic activity was not recognized.