抗菌性物質の化学的定量法 (第1報)

ジヒドロストレプトマイシンの比色定量法

抄録

It has been found that streptomycin and dihydrostreptomycin form sensitive orange red color with sodium pentacyanoamminferroate and potassium ferricyanide, on the addition of acetone, and that the relationship between the optical density of this coloration and the concentration of the streptomycin followed the Beer's law. This coloration was utilized in the colorimetric determination of dihydrostreptomycin. The determination was carried out with the ordinary dihydrostreptomycin sulfate as the standard. The potency obtained by this method and that from microbiological assay were found to agree. Potency determinations were carried out on the market products of dihydrostreptomycin.