抗菌性物質の化学的定量法 (第2報)

オキシテトラサイクリンの比色定量法

抄録

Determination of hydroxytetracyclin was found to be possible by the following method. A mixture of 1% ammonium molybdate solution and hydroxytetracyclin solution in an acetic acid buffer of pH 4 is heated at 60° for 15 minutes, shaken with an equal volume mixture of ethyl acetate and ether, and the aqueous layer is diluted to a definite volume. This aqueous solution is optically measured in a 2-cm. cell with S50 and S66 filters, and the value of E_S50-E_S66 is calculated.