無発熱性医薬品の製造に関する研究 (第6報)

Pyrogen並びにそのキノン処理の電気泳動的検討

抄録

The true nature of pyrogen and changes in the behavior of pyrogen after quinone treatment were studied through filter paper electrophoresis.
1) Electrophoresis of pyrogen-containing culture filtrate showed that pyrogen with positive TBP reaction was composed of electrically almost neutral portion (P_a) and positively charged one (P_β).
2) P_α is removed well by adsorptives and ion exchange resins but the removal of P_β is difficult. This fact well explains the observations (cf. previous paper) that some existing method of purifications were not necessarily effective in removing pyrogen.
3) The solution obtained by the treatment of pyrogen-containing culture filtrate with quinone and then activated carbon gives negative TBP reaction and such image is not detected by electrophoresis.
4) Electrophorectic examination of pyrogenic glucose injections gave the same results as those with the culture filtrate.