瀘紙微量電気泳動法による医薬品の分析 (第3報)

サリチル酸及びその誘導体の分離及び確認

抄録

In continuation of previous works, separation and determination of salicylic acid, acetylsalicylic acid, phenyl salicylate, sodium p-aminosalicylate, and salicylamide were attempted by paper electrophoresis. Migration values and conditions of each compound are indicated in Figs. 1-7. It was found that phenyl salicylate alone stayed in the original spot while the other four compounds exhibited the maximum difference in the migration distance at around pH 4.0, using any of the buffers. Detection of the migrated position was made by the Okuma's reagent, and the limit of detection was 5-10 γ. In the case of a mixture of these five compounds, as shown in Table I, the separatory detection of each is made as follows: Three kinds of sample solutions, 1% ethanolic solution, and treated with alkali and acid, are submitted to electromigration under the same conditions, and each compound is determined from its migration value, direction of migration, and coloration to the reagent. Okuma's reagent consists of 1% sodium nitroprusside, 1% hydroxylamine hydrochloride, and 1N sodium hydroxide, each of which is sprayed on an air-dried filter paper, in that order.