細菌代謝産物のコリンエステラーゼ活性に及ぼす影響 (第3報)

イカを培地とした場合の実験

抄録

Homogenate, extract, and proteins of a squid were used as a medium for the culture of various bacteria and the changes in pH, amount of volatile basic nitrogen, trimethylamine-nitrogen, and degree of cholinesterase inhibition of each culture filtrate with passage of time were measured. It was thereby found that both the pH and basic nitrogen increased with passage of time, while trimethylamine-nitrogen and cholinesterase inhibition reached the maximum after 48 hours and decreased by 72 hours later. There were no cholinesterase inhibition or the presence of trimethylamine-nitrogen when squid protein was used as the medium, while these were extremely high when the squid homogenate and extract were used as the culture medium. Further, there was a significant relativity between these. The inhibition of cholinesterase activity by the culture filtrate using squids must be due to the trimethylamine formed by the bacterial reduction of trimethylamine oxide contained in the squid. Liberation of histamine was also measured in some of these culture media but the amount was extremely minute.