飲食物防腐剤の研究 第67報
パラオキシ安息香酸エステル類の飲食物防腐剤としての研究 その5 Zygosaccharomyces japonicusの呼吸についての二, 三の基礎的研究
1957 年 77 巻 12 号 p. 1288-1292
詳細
1957 年 77 巻 12 号 p. 1288-1292
A few experiments were carried out on the respiration of Zygosaccharomyces japonicus by the use of Warburg manometer. Oxygen consumption was observed only when glucose was used as a substrate on the neutral side or acetic acid as the substrate on acid side, and no absorption of oxygen was detected with various organic acids in the Krebs cycle. This must be due entirely to the permeability through the cell membrane. Lyophilized preparation of the yeast showed activity of succinic dehydrogenase and the activity was found to be inhibited by malonic acid. Succinic dehydrogenase was extracted from Zygosaccharomyces japonicus and the activity of this cell-free preparation was not affected by sodium dehydroacetate (1×10-3M) and sodium dodecylsulf ate (1×10-4) in minimum concentration completely inhibiting the growth of this yeast, but was inhibited 50% by the same concentration (3×10-3M) of methyl p-hydroxybenzoate.
