蛇毒蛋白の研究 (第4報)
コプラ毒のアルカリ性フォスファターゼ類の分離について
1958 年 78 巻 4 号 p. 368-375
詳細
1958 年 78 巻 4 号 p. 368-375
The venom from Formosan cobra (Naja naja atra CANTOR), differing from that of domestic asp (Agkistrodon halys blomhoffi BOIE), is adsorbed easily on alumina-C γ-gel, calcium phosphate gel (Ca-P-gel), as well as carboxymethylcellulose (CM-cellulose), Amberlite IRC-50 (XE-64), and phosphate-cellulose, and any of the ion exchangers can be utilized for obtaining phosphodiesterase free from phosphomonoesterase from cobra venom. The sample of purified phosphodiesterase from the cobra venom does not contain 5′-nucleotidase or phosphomonoesterase, being approximately 25 times purer than the crude venom, and was obtained in approximately 10% yield.
Column chromatography with CM-cellulose was applied to the separatino of alkaline phosphatase from cobra venom and stepwise elution analysis and gradient elution analysis, using acetate buffer solution as the eluate, were attempted. By these means, glycerophosphatase, phosphodiesterase, and 5′-nucleotidase were separated in good yields. It was found that the active portion of phosphodiesterase was separated into two by this column chromatography and the presence of two kinds of phosphodiesterase was evidenced by rechromatography.
