臨床分析化学の基礎的研究 (第2報)

血漿中4-アセトアミドアンチピリンの比色定量

抄録

Colorimetric determination of 4-acetamidoantipyrine in plasma was studied and the standard procedure was established. Its principle is based on the oxidative condensation of 4-aminoantipyrine (Emerson's reagent), produced from 4-acetamidoantipyrine by hydrolysis, with phenols in weak alkaline medium to yield an indophenol-type pigment. Of the various kind of phenolic compounds, the simple phenol was found to be the most suitable for this purpose. It has also been found that development of the color is constant and stable over a wide pH range employing borate buffer already reported by Lacoste, et al. A known amount of 4-acetamidoantipyrine added to plasma was measured, using the calibration curve by the standard procedure and the results were quite satisfactory.