Abstract
Chromatographic separation of a combination of codeine and hydrocodeine was attempted in continuation of earlier works on codeine and ephedrine, codeine and methylephedrine, hydrocodeine and ephedrine, hydrocodeine and methylephedrine, and ephedrine and methylephedrine. The two bases were found to be separated quantitatively by the partition column chromatography over Celite, using phosphate buffer of pH 6.4 as the stationary phase and benzene-acetone mixture (50:1 by volume) and chloroform as the mobile phase.
