抗菌性物質の化学的定量法 (第15報)
クロラムフェニコールの比色定量法
1962 年 82 巻 3 号 p. 338-342
詳細
1962 年 82 巻 3 号 p. 338-342
Colorimetric determination of chloramphenicol in solution was carried out. To 1cc. of a sample containing chloramphenicol placed in a glass-stoppered test tube, 1cc. of Clark-Lubs buffer of pH 7 and 3cc. of isoamyl acetate are added, the mixture is shaken for 10 minutes, and centrifuged. To 2cc. of this supernatant, 3% isonicotinic acid hydrazide solution and 1cc. of 6% sodium hydroxide solution are added and the mixture is shaken at 30° for 30 minutes. The supernatant layer is removed and absorbancy of the lower colored layer is measured, using S-40 filter. A blank test is carried out at the same time in the same manner, using the same reagents but distilled water in place of the sample solution. Potency of the test solution is calculated from the calibration curve prepared with chloramphenicol of the known potency. This method was tested on samples left at room temperature or in a refrigerater, left as acid or alkaline solution, or boiled. The values so obtained were compared with those determined by the cup method and the values were found to agree well.
