抄録
Urogastrone was isolated from pregnant human urine by the sequential steps of benzoic acid adsorption, acetone fractionation, DEAE-cellulose chromatography, and gel filtration. The preparation so obtained was free from gonadotropin activity. Comparative examination of urogastrone isolated from pregnant and non-pregnant human urine by the present method and other known methods showed that the present method gave a preparation with high unit activity in a high yield, free from gonadotropin activity. The yield of purified urogastrone by this method was 1.5 mg/liter from pregnant urine and 1.3 mg/liter from non-pregnant urine, and their inhibitory activities were 0.25 and 1.0 mg/kg, respectively. Yield of total inhibitory activity from the pregnancy urine was approximately five times that from non-pregnancy urine by the present method while this was about three times by the known method. These facts indicate the high urogastrone content in the urine during pregnancy. The purified urogastrone obtained in the present experiment from pregnancy urine was found to be practically homogeneous by electrophoretic analysis, and is a high-molecular glycoprotein containing 20% of sugar, with an isoelectric point at pH 3.5.
