抄録
Gas chromatographic method for the determination of bile acids in bear gall was developed. Neutral lipid-free methanolic extract from bear gall was treated with 2.5 N sodium hydroxide in an autoclave. Bile acids thus obtained were esterified by diazomethane and then their hydroxyl groups converted to trimethylsilyl ethers in anhydrous pyridine. Gas chromatography using 0.5% cyclohexanedimethanol succinate column resulted in the quantitative separation of cholanic acid derivatives. The calibration curve was constructed with the use of lithocholic acid as an internal standard.
