Limulus Lysate(Pre-gel)のゲル化反応に関する研究(第3報)各種物質のゲル化反応に及ぼす影響

抄録

Effect of nucleic acid-related substances, amino acids, saccharides, protein-denaturing or -precipitating agents, SH reagents, reducing agents, biological products, urine, and saliva, on the gelation reaction of limulus lysate was examined. Most of them have no effect on the gelation reaction, but some inhibition was observed in benzyl alcohol, ATP of nucleic acid-related substances, cysteine, and mercaptoethanol among SH reagents. Protein-denaturing agents inhibited the gelation reaction at the concentration higher than a certain level. Saliva, urine, and biologicd products showed no inhibition of the gelation reaction. It was also found that the limulus test was applicable to the detection of endotoxin in products at various stages of manufacturing process.