抄録
An improved method for microassay of blood cholinesterase activity is described. The method is an application of fluoromotric titration for acetic acid. The reaction mixture, composed of 100 μl of substrate, 50 μl of Tris buffer (pH 7.6), and 50 μl of sample, is incubated for 10-20 min at 30°, and 1000 μl of umbelliferone (10-6M)-isopropanol is added to the mixture. After centrifugation, 1000 μl aliquot of isopropanol supernatant, containing 0.2-15 μmoles of acetic acid, is titrated by fluorometry with 0.02N isopropanolic NaOH. Linearity of the reaction rate is obtained within 40 min of incubation. This method is applicable to colored and insoluble materials such as erythrocyte, in the same way as in the case of serum.
