ガスクロマトグラフィーによるtRNA中の5-Methylcytosineの分析

抄録

Gas chromatographic analysis of 5-methylcytosine in t-ribonucleic acid (tRNA) is reported. This method involves hydrolysis of tRNA with perchloric acid, neutralization with CG-4B resin (OH-form), selective elution of cytosine and 5-methylcytosine from Dowex 1 X2(HCOO- form), iodination of cytosine, trimethylsilylation, and injection into a gas chromatograph. This method requires only a few milligrams of tRNA, and can be performed within one day. Application of this method for the analysis of 5-methylcytosine in DNA was suggested. Analytical results of 5-methylcytosine in tRNAs from several sources indicated that tRNA extracted from tumor tissues contain less 5-methylcytosine than that from normal tissues.