拡散理論の"帯培養法"への応用(第1報)理論

抄録

The mechanism of the microbiological assay was interpreted as the diffusional mass transfer of the drug starting from the drug phase, travelling through the receptor phase, reacting with bacteria, death and growth of bacteria in the receptor phase, and the show-up of clear zone. Simulation technique using an electronic computer can be applied to the calculation of the drug concentration and the concentration (number) of bacteria in the receptor phase. If the same diffusional medium is used in the drug and the receptor phase, the drug concentration at the drug-receptor interface may be maintained constant (C₀/2) in the linear diffusion system. This phenomenon can be applicable to the determination of the minimum growth inhibitory concentration (MIC). In a two-dimensional diffusion system, the drug concentration at the drug-receptor interface is not constant theoretically, then the system is not applicable to the determination of MIC. In general, the two-dimensional diffusion system gives a shorter clear zone length than in the linear diffusion system. This phenomenon may be attributed to the lower drug concentration at the interface than in the linear diffusion system.