ろ紙上におけるヘミンとヘモグロビンヘムの安定性に対する日光と紫外線の影響
1977 年 97 巻 2 号 p. 191-196
詳細
1977 年 97 巻 2 号 p. 191-196
Effect of sunlight and ultraviolet ray on the stability of hemin and hemoglobin heme, which were stained on a filter paper, was studied by the combined use of paper chromatography and spectrophotometry. Hemin and hemoglobin heme decreased rapidly by irradiation with sunlight in summer (maximum illuminance, 60000 lx) or with ultraviolet ray (250-400 nm) within 1-5 hr. Hemin which was allowed to stand in two places in a room (maximum illuminance 10000 and 250 lx) decreased markedly with lapse of time up to 25 days, whereas hemoglobin heme was more stable than hemin under the same conditions. Both hemin and hemoglobin heme were fairly stable in a dark place for about 40 days. A stable greenish yellow brown spot (Rf 0.00), which was insoluble in many organic solvents, acids, and alkalis, was produced from the stains of hemin and hemoglobin heme by irradiation with sunlight or with ultraviolet ray. A similar substance was also produced from hemin and hemoglobin heme when allowed to stand in the room.
