Effect of Sodium Copper Chlorophyllin on Lipid Peroxidation. I. Effect on Lipid Peroxidation in Rat Liver Homogenates in the Presence of Both Fe²⁺ and L-Ascorbic Acid

Abstract

Effect of sodium copper chlorophyllin (SCC) on the lipid peroxidation in rat liver homogenate was examined. Formation of lipid peroxides was estimated by the thiobarbituric acid (TBA) method, and SCC was found to inhibit the increases of TBA value caused by both ferrous ion and L-ascorbic acid. The ferrous ion and ascorbic acid-stimulated lipid peroxidation in liver homogenates was also expressed as the increase in the oxygen consumption of liver homogenates. SCC repressed the acceleration of oxygen consumption of liver homogenates by both ferrous ion and ascorbic acid. The antioxidative effect of SCC was thought to be attributed neither to free cupric ion nor to pro-oxidative action of SCC on ascorbic acid.