Studies on the Quality Estimation of Drugs of Animal Origins. I. The Determination of Bufadienolides in Ch' an Su (Senso) and Its Preparations

Abstract

The quality estimation of Ch' an Su (Senso) based on the chromatographic determination of three major bufadienolides (resibufogenin, cinobufagin, and bufalin) was investigated. Reliable methods established by the use of gas-liquid chromatography, high performance-liquid chromatography, and thin-layer densitometry were found to be routinely applicable for bufadienolide determination of "Rokushingan, " a commercial drug containing.