The purpose of this study was to investigate the antibacterial effect after curing of dentine primer incorporating antibacterial monomer methacryloyloxydodecylpyridinium bromide (MDPB). Cured specimens were fabricated in the mould with 5% MDPB-containing primer or control primer, adhesive, and composite. Elution of antibacterial components and inhibitory effect against bacterial growth of cured specimen were determined by agar disc diffusion method. Bactericidal activity of cured primer was evaluated by counting the number of
Streptococcus mutans after contact with the specimen for 1 hour and the elution of uncured MDPB was measured. Although experimental and control primers produced no inhibition zones against
Streptococcus mutans and
Lactobacillus casei, bacterial growth on agar under specimen of MDPB-containing primer was inhibited compared with controls. Both primer produced inhibition zones against
Actinomyces viscosus, the size of halo by experimental primer being greater than control. Significantly less bacteria was recovered after contact with experimental primer compared with control, but elution of uncured MDPB was less than the minimum inhibitory concentration value. It can be concluded that dentine primer incorporating MDPB shows inhibitory and bactericidal activity which depend upon immobilized agent even after curing, although a small amount of uncured MDPB eluted also may somewhat inhibit susceptible species such as
Actinomyces viscosus.
View full abstract