Screening of the fibrinolytic enzymes from Basidiomycetes was investigated. Twelve strains of cultivated mushrooms and 43 strains isolated from wild mushrooms were used in this study. Fibrinolytic activities of mycelial culture broth of each strain were determined. The broths of Tamogitake (Pleurotus corenucopiae) and a wild strain (W510), showed remarkable fibrinolytic activities. Both enzyme activities were very similar to that of a plasminogen type enzyme, and they dissolved human fibrin as the same extent with bovine fibrin. Further, both enzyme activities were inhibited by addition of PMSF, which is a serine proteinase inhibitor. PCMB (a SH inhibitor) inhibited the activity of Tamogitake, but not for the activity by W510. The activity in the Tamogitake broth was unstable, but that in the W510 was stable even stored for twelve months at 0-10℃, and recovered 90 % of the initial activities after storing for ten months at room temperature.

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