The effect of nucleic acid-related compounds on sawdust-based cultivation of Lentinula edodes was examined. Ribonucleic acid (RNA-M) and 5'- nucleotide mixture (RNA-Nt) were used as the nucleic acid-related compounds. Nucleic acid-related compounds were added to the medium according to the following 2 methods: (1) Adding the nucleic acid-related compounds to the water at the medium preparation. (2) Soaking the medium in the nucleic acid-related compounds aqueous solution. In method (1), the fruit body yield increased and the fruit body enlarged. In method (2), the fruit body was enlarged by RNA-Nt. Next, using cultural facilities of the shiitake cultivator and the sawdust-based substrate, the fruit body yield was examined by addition to the medium preparation of RNA-M. Yield increased and the fruit body enlarged at 0.73% addition of the medium on oven dry weight basis. At 1.46% addition, the medium surface was contaminated by mold, and the fruit body yield decreased.

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"Wakame" seaweed dry powder was made of non-food parts of "Wakame" (Undaria pinnatifida) by the spray drying sodium carbonate dissolution method. The effects of "wakame" seaweed dry powder on the mycelial growth of four species of edible mushrooms (Lentinula edodes, Glifola frondosa, Pleurotus eryngii, Hericium erinaceum) and on fruit body development of L. edodes were examined. The mycelial growth was measured in liquid medium, agar medium and sawdust-based substrate containing "wakame" seaweed dry powder. Mycelial growth increased in four species of edible mushrooms in each medium. In the sawdust-based substrate, L. edodes showed the highest mycelial growth, an increase in yield and enlargement of the fruit body at 0.17% additive rate to the absolute dry weight substrate. "Wakame" seaweed dry powder is effective for the improving yield and quality of the fruit body of L. edodes in sawdust-based substrate.

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The effect of nucleic acid related compounds on the mycelial growth of edible mushrooms were examined. Ribonucleic acid (RNA-M) and 5'-nucleotide mixture (RNA-Nt) were tested for Lentinula edodes, Pholiota nameko, Glifola frondosa, Hypsizygus marmoreus and Pleurotus eryngii. RNA-M and RNA-Nt were useful additives for all 5 species used in the experiments. RNA-Nt was more effective in the liquid medium. In contrast, RNA-M resulted higher effect in the agar and sawdustbased substrate. Maximum growth was observed at 0.5% addition on the agar medium and 1.0% addition in the sawdust-based medium, respectively. Growth index was calculated to be 141 and 151 in L. edodes at the most effective additional ratio on respective media.

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